anti py stat1 Search Results


anti py stat1  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti py stat1
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Anti Py Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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py-stat1 (y701)  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc py-stat1 (y701)
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Py Stat1 (Y701), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/py-stat1 (y701)/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
py-stat1 (y701) - by Bioz Stars, 2026-03
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rabbit monoclonal anti py stat1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit monoclonal anti py stat1
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Rabbit Monoclonal Anti Py Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
rabbit monoclonal anti py stat1 - by Bioz Stars, 2026-03
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anti py 701 stat1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti py 701 stat1
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Anti Py 701 Stat1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
anti py 701 stat1 - by Bioz Stars, 2026-03
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mouse anti py stat1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc mouse anti py stat1
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Mouse Anti Py Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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anti-py(701)stat1 conjugated alexafluor 647  (Becton Dickinson)
90
Becton Dickinson anti-py(701)stat1 conjugated alexafluor 647
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Anti Py(701)Stat1 Conjugated Alexafluor 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-tyrosine-phosphorylated stat1 anti-py-stat1  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc anti-tyrosine-phosphorylated stat1 anti-py-stat1
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Anti Tyrosine Phosphorylated Stat1 Anti Py Stat1, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphotyrosine 690-stat2 (pystat2) antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc phosphotyrosine 690-stat2 (pystat2) antibody
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Phosphotyrosine 690 Stat2 (Pystat2) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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py-stat-1  (Becton Dickinson)
90
Becton Dickinson py-stat-1
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Py Stat 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho stat3  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti phospho stat3
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Anti Phospho Stat3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphotyrosine (py)stat1 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc phosphotyrosine (py)stat1 antibody
Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with <t>anti-STAT1</t> (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), <t>anti-pY-STAT1</t> (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.
Phosphotyrosine (Py)Stat1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with anti-STAT1 (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), anti-pY-STAT1 (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.

Journal:

Article Title: Adjuvant Activities of Novel Cytokines, Interleukin-23 (IL-23) and IL-27, for Induction of Hepatitis C Virus-Specific Cytotoxic T Lymphocytes in HLA-A*0201 Transgenic Mice

doi: 10.1128/JVI.78.17.9093-9104.2004

Figure Lengend Snippet: Expression and activity of the scIL-12, scIL-23, and scIL-27 fusion proteins. (A) p3XFLAG-IL-12 (IL-12), p3XFLAG-IL-23 (IL-23), p3XFLAG-IL-27 (IL-27), and p3XFLAG (vector) were transiently transfected into 293T cells. Proteins secreted in the supernatants were immunoprecipitated with the anti-FLAG antibody. The immunoprecipitated proteins were separated on by SDS-12% polyacrylamide gel electrophoresis and subjected to Western blotting analysis with the anti-FLAG antibody. The positions of protein molecular mass markers (in kilodaltons) are shown in the figure, and the three arrows indicate the bands of the scIL-12, scIL-23, and scIL-27 fusion proteins. (B to D) STAT tyrosine phosphorylation assay. 293T cells were transiently transfected with either pME18S-IL-12Rβ1 plus pME18S-IL-12Rβ1 (B), pME18S-IL-12Rβ1 plus p3XFLAG-IL-23R (C), or p3XFLAG-WSX-1/TCCR plus p3XFLAG-gp130 (D). After 36 h, the cells were stimulated for 45 min with the culture supernatant containing either scIL-12 (IL-12) (B), scIL-23 (IL-23) (C), or scIL-27 (IL-27) (D) at final concentrations of 0, 0.5, 5, and 50%. As negative controls, the cells were stimulated for 45 min with the culture supernatant containing either scIL-23/scIL-27 (B), scIL-12/scIL-27 (C), or scIL-12/scIL-23 (D) at a final concentration of 50%. The cells were then subjected to Western blotting with anti-STAT1 (Total STAT1) (D), anti-STAT4 (Total STAT4) (B and C), anti-pY-STAT1 (pY-STAT1) (D), and anti-pY-STAT4 (pY-STAT4) (B and C) antibodies.

Article Snippet: The cells were then subjected to Western blotting with anti-STAT1, anti-STAT4 (Santa Cruz Biotechnology, Santa Cruz, Calif.), anti-pY-STAT1 (Cell Signaling Technology, Inc., Beverly, Mass.), or anti-pY-STAT4 (Zymed Laboratories, Inc., South San Francisco, Calif.) antibody.

Techniques: Expressing, Activity Assay, Plasmid Preparation, Transfection, Immunoprecipitation, Polyacrylamide Gel Electrophoresis, Western Blot, Phosphorylation Assay, Concentration Assay